ADVANCED FEATURES
PerkinElmer Life and Analytical Sciences 145
To establish the optimal region settings using the Reverse Region option, you will
first need to approximate these settings using the Sample Priostat Preview
function:
1. Load your sample(s) into a cassette. Attach the Priostat flag in the reset
position to the cassette.
2. Preview your samples using one of the Sample Priostat Preview options
(Normal, Alpha Beta or Low Level). The appropriate option corresponds to the
count mode that will be used to count the samples. All of these options are
available via the Run-Sample Priostat menu option. The Sample Priostat
window is displayed.
3. Select Count from the Run menu. Counting will commence after the instrument
identifies the Priostat flag.
4. Allow the samples to count to a statistical accuracy (2S%) of 1.0 (equivalent to
40,000 gross counts). While previewing the sample, determine the lower level
and upper level region settings by observing the sample spectrum.
5. Stop the Sample Priostat count via the Run-Stop menu option. Select Next
Sample from the Run menu, if you would like to count additional samples.
6. Select Reverse Region from the Run menu. The Sample Priostat window is
displayed. Enter the region settings established during the preview count.
7. Select Count from the Run menu.
8. Stop the Reverse Region sample counting using the Run-Stop menu item.
9. Select Next Sample followed by Count from the Run menu, if you would like to
count additional samples.
10. Stop the Reverse Region sample counting by selecting the End Sample Priostat
menu item. An output window is displayed which provides you with the
Equivalent Unquenched Settings for Regions A, B and C for the sample nuclide.
11. Add this nuclide with the optimized region settings into the Sample Nuclide
Library for later use in various assays. Any quench standards sets defined for
use with this nuclide will automatically use the new region settings. No new
quench standards need to be counted as long as the nuclide is the same.