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Sakura Tissue-Tek Xpress x120 - User Manual

Sakura Tissue-Tek Xpress x120
82 pages
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Operating
Operating
Manual
Manual
Rapid Tissue Processor
Tissue-Tek
®
Xpress
®
x120

Table of Contents

Other manuals for Sakura Tissue-Tek Xpress x120

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Summary

Introduction

Intended Use

Instrument for processing human and animal tissue specimens for histopathology examination.

General Description

Describes the Tissue-Tek Xpress x120 as a continuous rapid tissue processor.

Safety Precautions

Defines safety labels (NOTE, CAUTION, WARNING) and lists specific safety instructions.

Cabinet Front Overview

Details components of the instrument's front cabinet, including access doors and indicators.

Front Cabinet Components and Indicators

Details indicators, doors, cabinets, and feet/casters on the instrument's front.

External Interface and Controls

Describes power switch, start button, USB interface, and alarm/UPS ports.

Location of Major Systems and Components

Identifies major systems and components of the instrument via a diagram.

Loading Station and Microwave Stations

Describes the loading station and microwave retorts (#1 and #2).

Preheating Chambers and Reagent Cabinet

Details preheating chambers and the cabinet for storing reagent containers.

Vacuum Stations and Paraffin Oven

Describes vacuum stations (#3 and #4) and the paraffin oven for reagent heating.

Overflow Bottles and Unloading Station

Details overflow bottles and the station for storing processed baskets/magazines.

Transfer System and Fume Control System

Explains the automated transfer system and the fume control system.

Control Panel Overview

Details the controls and indicators for operating the instrument.

Instrument Specifications

Lists models, power requirements, ratings, noise level, and fume control compliance.

Safety Standards and Compliance

Lists electrical safety certifications and compliance standards for various models.

Section 2 Installation

Powering the Instrument

Describes how to turn the instrument's main power on and off.

Installation and Environmental Factors

Covers installation requirements and environmental factors for optimal performance.

Reagent Bottle Setup and Pre-Start Checks

Instructions for setting reagent bottles and performing daily pre-start checks.

Unpacking and Accessories

Lists accessories provided and how to handle missing or damaged items.

Section 3 Customization of Settings

General Information and UTILITIES Menu Access

Explains system customization and how to access the UTILITIES menu.

System Setup Options

Allows configuration of user-selectable options and operating parameters.

User Account Management

Covers PASSWORDS and USER DEFINED FILE for managing system users and data.

Automated Operation Settings

Covers READY/SHUTDOWN TIMES and PROCESS RECOVERY functions.

System Setup Parameters (Part 1)

Details settings for UPS, OUTPUT, EDIT ITEM, FILE NAME, SOUND VOLUME, SOUND SELECTION.

System Setup Parameters (Part 2)

Details settings for LANGUAGE, DATE FORMAT, TIME FORMAT, and DATE/TIME.

Setting User Names and Passwords

Explains how to set institution name, add/edit/delete user names and passwords.

Managing the User Defined File

Describes how to create identifiers for sorting data in Run History and Statistics.

Setting Ready and Shutdown Times

Sets automatic warm-up (ON) and shutdown (OFF) times for the system.

Setting Days Off

Overrides on/off time settings for specific dates when the instrument is not used.

Section 4 Operating Instructions

Initial Setup and Control Panel Display

Ensures initial setup is done; explains control panel display for operation.

Process Monitor Screen Overview

Displays system status during ready and processing states with various indicators.

Basket Preparation for Processing

Details using standard baskets or Paraform magazines for processing.

Tissue Specimen Preparation

Discusses specimen preparation procedures for the instrument.

System Start-Up Procedures

Explains how the instrument starts up and enters the WARMING UP SYSTEM cycle.

Auto Start and Manual Start

Details automatic and manual instrument start-up procedures.

System Warm-Up and Logging On

Explains the warm-up cycle and how to log into the system.

Logging Off and System Operation Phases

Recommends logging off and outlines the four phases of system operation.

Setting Onboard Preprocessing Times

Allows administrators to change preprocessing times for the loading station.

Loading Tissue Specimens and Initiating Processing

Steps to load specimens and start a processing cycle using the control panel.

Process Monitoring Details

Explains how the Process Monitor screen displays status and provides information.

Unloading Processed Specimens

Procedures for unloading processed baskets and transferring them.

System Shutdown Procedures

Details the shutdown process for efficiency, saving power and reagents.

Auto Shutdown and Manual Shutdown

Details automatic and manual instrument shutdown procedures.

Special Conditions and Considerations

Discusses process scheduler, reagent monitoring, and door open protocols.

Emergency Stop and Viewing Reports

Performing an emergency stop and accessing historical/statistical reports.

Software Version and Run History

Viewing instrument software versions and historical processing run data.

Viewing Statistics

Accesses statistical data on the number of runs and cassettes processed.

Viewing the Error Log

Accesses a log of errors occurring in systems, subsystems, or processes for diagnosis.

Section 5 Recovery Process

Overview and Detecting Errors

Describes recovery for operations suspended due to errors and how errors are detected.

Reprocessing in Retorts 3 or 4

Details reprocessing specimens starting in Retorts 3 or 4 after an error.

Section 6 Consumables and Accessories

Consumable and Standard Accessory Items

Lists consumable materials and standard accessory items with product codes.

Maintenance Items and AutoTEC Accessories

Lists maintenance items and accessories compatible with the AutoTEC Embedding System.

Ordering Information

Provides contact information for ordering consumables and accessories.

Section 7 Care of the Instrument

General Information and On Condition Maintenance

Information on instrument care and maintenance performed as needed.

Basket and Magazine Cleaning Methods

Details methods for cleaning processing baskets and magazines to prevent clogging.

Overflow Bottle and Pre-Processing Solution Replacement

Procedures for cleaning overflow bottles and replacing pre-processing solution.

Reagent Replacement Procedures

Explains when and how to replace reagents in Retorts #1 and #2.

Paraffin Replacement Procedures

Detailed steps for replacing paraffin in Retorts #3 and #4.

Unloading Station Paraffin and Reagent Counter Clearing

Procedures for replacing paraffin and clearing the reagent counter.

Daily Maintenance: Drip Tray Liner Replacement

Procedure for daily replacement of soiled drip tray liners.

Weekly Maintenance: Exterior and Retort Cleaning

Guides for cleaning the instrument exterior and retort interiors.

Retort Mesh Filter Cleaning

Instructions for cleaning retort mesh filters to prevent debris accumulation.

Paraffin Oven Mesh Filter Cleaning

Instructions for cleaning paraffin oven mesh filters to ensure proper filling and draining.

Retort Sight Tube Cleaning

Weekly cleaning of sight tubes to ensure proper reagent levels and prevent overflow.

Preheating Chamber Sight Tube Cleaning

Weekly cleaning of preheating chamber sight tubes for proper reagent levels.

Monthly Maintenance: Fume Control System Filter Replacement

Instructions for monthly replacement of the activated carbon filter for fume control.

Routine Maintenance Check List, Daily

Routine Maintenance Check List, Weekly

Section 8 Troubleshooting

General Troubleshooting Procedures

General advice and cautions for troubleshooting instrument errors.

Troubleshooting Chart

Lists common problems, probable causes, and remedies for the instrument.

Monitored Errors and System Behavior

Explains how errors are monitored, trigger alarms, and are logged.

Section 9 Service and Replacement Parts

Service Information and Contact

Guidance for contacting customer support and recording instrument details.

Replacement and Service Parts

Lists replacement, service, and consumable parts with product numbers.

Optional Accessory Items and Ordering

Lists optional accessories and provides ordering information.

Appendix: Rapid Tissue Processor Applications Manual

Overview of Applications Manual

Introduces the manual's purpose and guidelines for tissue processing.

Tissue Preparation Programs

Discusses immersion time settings (2mm or 3mm) for tissue processing.

Pre-Processing Solution and Fixative Use

Details the use and benefits of Pre-Processing Solution and Fixative.

Extended Immersion Program and Specimen Loading

Details the extended program and specimen loading process.

Molecular Fixative Usage

Explains the mandatory use of Molecular Fixative for specific studies like DNA, RNA, and protein.

Staining and Fixative Effects (H&E, IHC, RNA, DNA, Protein)

Compares staining results and effects of Molecular Fixative on various tissue studies.

RNA Tissue Processing Cleaning Instructions

Provides procedures and materials for an RNase-free cleaning environment.

Microtomy and Staining Considerations

Discusses sectioning and staining of processed tissue blocks.

Microscopic Evaluation and Reprocessing

Notes artifacts and provides procedures for reprocessing tissue.

Reagent Replacement Schedule

Recommends reagent replacement frequency based on cassettes processed or days.

Tissue Processing Dos and Donts

Lists recommended practices and prohibitions for tissue processing.

Processing Solutions Disposal

Refers to MSDS sheets for proper disposal of processing solutions.

Processing Flow Charts (Standard & Extended)

Outlines the steps for processing tissues using Standard and Extended Programs.

Sakura Tissue-Tek Xpress x120 Specifications

General IconGeneral
BrandSakura
ModelTissue-Tek Xpress x120
CategoryComputer Hardware
LanguageEnglish