2-2 Model 310 Series DH Incubator Thermo Scientific
Section 2
Calibration
1. Press the Mode key until Cal indicator lights.
2. Press the right arrow until “TEMPCAL XX.X” appears in the message
center.
3. Press up/down arrow to match display to calibrated instrument.
4. Press Enter to store calibration.
5. Press the Mode key to return to Run or the right/left arrow to go to
next/previous parameter.
Models 310, 311, 350 and 351 have a thermal conductivity (T/C) CO
2
sensor. Thermal conductivity of the incubator atmosphere is not only
effected by the quantity of CO
2 present, but also by the air temperature
and water vapor present in the incubator atmosphere. In monitoring the
effects of CO
2, air temperature and absolute humidity must be held
constant so any change in thermal conductivity is caused by a change in
CO
2 concentration.
Changing temperature or changing from elevated humidity to room
ambient humidity levels will necessitate a re-calibration of the CO
2
control.
T/C CO
2 Sensor Stabilization Periods
Some T/C CO
2 sensors go through an aging period, especially on new
installations. Calibration should be checked on a weekly basis, and
adjusted as necessary. When stabilization occurs, checks may become less
frequent.
Start -Up - The CO
2 sensor has been calibrated at the factory for 37°C
and elevated humidity. Allow the temperature, humidity, and CO
2 levels
in the chamber to stabilize at least 12 hours before checking the CO
2
concentration with an independent instrument.
Presently Operating - Make sure the chamber doors are closed. Allow at
least 2 hours after the temperature and CO
2 displays reach their setpoints
for chamber atmosphere stabilization.
Calibrating the T/C
CO
2 System
Calibrating Temp
(continued)
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