Model 310 Series DH Incubator 2-3Thermo Scientific
Section 2
Calibration
1. Make sure the stabilization periods outlined above are followed.
2. Sample the chamber atmosphere through the sample port with an
independent instrument. Sample the atmosphere at least 3 times to
ensure accuracy of the instrument.
3. Press the Mode key until the Cal indicator lights.
4. Press the right arrow until “CO2 CAL XX.X” is displayed in the
message center.
5. Press the up /down arrows to change the display to match the
independent instrument.
6. Press Enter to store the calibration.
7. Press the Mode key to return to Run or the right or left arrows to go
to the next/ previous parameter.
Models 320, 321, 360 and 361 have an infrared (IR) CO
2 sensor. Infrared
CO
2 sensors are not effected by chamber atmosphere temperature or
humidity. However, the light detector in the sensor is effected by wide
temperature changes. Therefore, changing temperature setpoints could
necessitate a recalibration of the CO
2. Chamber temperature should be
allowed to stabilize before checking CO
2 concentrations with an
independent instrument, especially on start-up.
All models equipped with an IR/CO
2 sensor have an automatic calibration
which occurs every 24 hours and lasts for 5 to 6 minutes. During
automatic calibration, the CO
2 display is blanked out and HEPA filtered
room air is pumped through the CO
2 sensor. A new CO2 calibration value
is stored in memory for use as the 0.0% CO
2 reference point. The keypad/
control panel is locked during calibration, with the message center reading
“CO2 AUTO CAL”.
IR CO
2 Sensor Stabilization Times
Start-Up- Allow the temperature and the CO
2 of the cabinet to stabilize at
least 12 hours before proceeding.
Presently Operating - Allow CO
2 to stabilize at least 2 hours at setpoint
before proceeding.
Calibrating the
Infrared CO
2 System
Calibrating T/C CO2
System (continued)
To Next Page
Loading...
