YOKOGAWA CQ1 - Page 22

1-2 <1. Function Overview>
IM 80J01A01-01E
Measurement values
CQ1 outputs the following measurement values by analyzing confocal
images of fluorescently labelled each cell.
Measurement
Unit
Measurement
Method
Description
Morphological
features
Area
µm
2
2D
measurement
Measure the area of objects.
Circumference
µm
2D
measurement
Measure the circumference of object.
Circularity
-
2D
measurement
Compare the area of the circumscribed
circle and the area of the object.
Circularity is expressed by a positive
number of less than 1.0, and perfect circle
is 1.0
Volume
µm
3
3D
measurement
Measure total volume of objects.
Surface area
µm
2
3D
measurement
Measure total surface area of objects.
Sphericity
-
3D
measurement
Compare the volume of an intracellular
structure and its circumscribed globe.
Sphericity is expressed by a positive
number of less than 1.0, and perfect globe
is 1.0.
Diameter
µm
2D
measurement
Measure diameter of the circumscribed
circle of an object.
3D
measurement
Measure diameter of the circumscribed
globe of an object.
Intensity
Sum
-
2D/3D
measurement
Measure the sum of pixel intensity of
objects.
Average
-
2D/3D
measurement
Measure average pixel intensity of
objects.
Standard
deviation
-
2D/3D
measurement
Measure standard deviation of pixel
intensity of objects.
Minimum
-
2D/3D
measurement
Measure minimum pixel intensity among
objects.
Maximum
-
2D/3D
measurement
Measure maximum pixel intensity among
objects.