<7. Trouble Shooting> 7-5
IM 80J01A01-01E
Probable Cause / Corrective Action
The laser autofocus does not operate
properly.
Kind of sample vessel might be
wrong.
Confirm that sample vessel
corresponds to that of designated
in measurement protocol.
It might be that sample vessel which
is not registered is used.
Register the sample vessel (refer
to page 5-19)
Use registered sample vessel
Is gel used for cell culture?
In “Sample Vessel Type” of
Sample window, check “Laser AF
Bottom Detector”. (Refer to page
5-19)
Is sample vessel whose cell culture
surface is processed especially?
In “Sample Vessel Type” of
Sample window, check “Laser AF
Bottom Detector”. (Refer to page
5-19)
In case of measuring other sample
in same protocol again, please
load the protocol in each time.
The Image autofocus does not
operate properly.
It might be that sample is too dark or
bright dusts exist in sample vessel.
Uncheck “ImageAF”
Quality of map image is bad
Zoom map image, and map image
is shown in original resolution
Despite focusing by “Search”
function, images acquired by “Rec”
are defocused.
In case that “Rec” position is far from
“Search” position, Z position that
sample exist may different.
Perform focusing by “Search” at
“Rec” position.
Waiting occurs during the
measurement.
It might occur in case when image
acquisition and heavy duty analysis
are processed concurrently.
It is recommended that image
acquisition and analysis are
processed separately.
It might be that imaging Area is not
set.
Set imaging Area.
It might be that well for “Rec” isn’t
selected.
Select well for “Rec”.
The error occurs just after “Rec”
button being clicked.
It might be that the parameter(s) set
in analysis is (are) not appropriate.
Reset the parameters.
It might occur that memories of
workstation run out because of heavy
duty analysis.
Reset protocol.
It might occur that memories of
workstation run out because of too
many number of Z stack.
Reduce the number of Z stack.
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