OPERATION
Axio Observer Illumination and contrast techniques ZEISS
12/2016 431004-7244-001 147
• Set the light intensity to a comfortable level by using the illumination control wheel (Fig. 148/32).
• Close the luminous-field diaphragm
(Fig. 148/10) until it is visible in the field of view
(not necessarily in focus) (Fig. 149/A).
• Bring the edge of the luminous-field diaphragm
into focus (Fig. 149/B) by adjusting the height
of the condenser (Fig. 148/13).
• Center (Fig. 149/C) the luminous-field
diaphragm using the centering screws
(Fig. 148/8) and open until the edge of the
diaphragm just disappears from the field of
view (Fig. 149/D).
• To set the aperture diaphragm, remove one eyepiece from the eyepiece tube and adjust the aperture
diaphragm of the condenser to approximately 2/3 the diameter of the exit pupil of the eyepiece
(Fig. 149/E).
The settings required for optimum contrast depend on the specimen.
• Reinsert the eyepiece and where necessary refocus on the specimen using the fine focusing drive.
• Adjust the light intensity using the rocker switch.
The field size and objective aperture change each time the objective is changed, so that for
optimal results the luminous-
field diaphragm and aperture diaphragm should be readjusted
whenever the objective is changed.
Fig. 149 Diaphragm settings in transmitted
light brightfield acc. to KÖHLER
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