Zeiss Axiotech - Operation

Axiotech and Axiotech
vario
Carl Zeiss
B 40-020 e 06/99 3-1
OPERATION
Contents
3 OPERATION ................................ ................................ ................................ ................... 3-3
3.1 Overview of operation and function controls ................................ ................................ ....3-3
3.1.1 Operation and function controls of the Axiotech 30 ................................ ......................... 3-3
3.1.2 Operation and function controls of the Axiotech 100 ................................ ....................... 3-4
3.1.3 Operation and function controls of the Axiotech
vario
100................................ .................... 3-5
3.2 Switch on the microscope................................ ................................ ................................ 3-6
3.2.1 Switch on the Axiotech 30 and Axiotech 100 ................................ ................................ ...3-6
3.2.2 Switch on the Axiotech
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100 (separate SNT 12 V DC, 100 W power unit, stabilized) .......3-6
3.3 Setting the microscope (basic settings) ................................ ................................ ............. 3-7
3.4 Illumination and contrasting techniques ................................ ................................ ........... 3-7
3.4.1 Setting of reflected-light brightfield for KÖHLER illumination ................................ ............ 3-7
3.4.2 Setting of reflected-light darkfield................................ ................................ .................. 3-10
3.4.3 Setting of reflected-light interference contrast................................ ................................ 3-12
3.4.4 Setting of reflected-light polarization contrast ................................................................ 3-15
3.4.5 Setting of epi-fluorescence................................ ................................ ............................. 3-17
3.4.6 Change of filters in the reflector slider................................ ................................ ............ 3-21
3.4.7 Setting of transmitted-light brightfield for Köhler illumination................................ ......... 3-22
3.4.8 Setting of transmitted-light polarization contrast ................................ ............................ 3-24
3.4.9 Setting of transmitted-light phase contrast ................................ ................................ .....3-29
3.4.10 3D Microscopy ................................ ................................ ................................ .............. 3-30
3.5 Attachment of photomicrography equipment................................ ................................ .3-31
3.5.1 Attachment of SLR camera, e.g. CONTAX Aria ................................ ............................... 3-31
3.5.2 Attachment of MC 80 DX microscope camera (35 mm format with film cassette 35 mm) 3-33
3.5.3 Attachment of MC 80 DX microscope camera (large format with 4 × 5" large-frame
attachment)................................ ................................ ................................ ................... 3-34
3.6 Attachment of videomicroscopy equipment................................ ................................ .... 3-35
3.6.1 Selection and assembly of adapters for video cameras ................................ .................... 3-36
3.6.2 Attachment of video camera................................ ................................ .......................... 3-37
3.7 Measuring, counting and comparing techniques using the microscope ........................... 3-38
3.7.1 Length measurements using stage micrometer and crossline micrometer ........................ 3-38
3.7.2 Length measurement using the digital eyepiece measurement device.............................. 3-39
3.7.3 Determination of microhardness using the MHT-4 microhardness tester.......................... 3-42
3.7.4 Determination of grain sizes in material structures ................................ ......................... 3-43