Axiotech and Axiotech
vario
Carl Zeiss
B 40-020 e 06/99 3-11
• Remove any light and/or attenuation filter which might be in the beam path, i.e. pull out the filter
slider (3-10/3) completely or switch to the free light path position.
• Push reflector slider HD or HD DIC (3-10/7 or 6) into the reflected-light illuminator until the darkfield
position is switched on (D mark visible on the reflector slider).
• Open the luminous-field diaphragm (3-10/1) and the aperture diaphragm (3-10/2) completely.
• Turn illuminance control (3-10/4) to the right stop position (full lamp voltage); if required, match the
brightness to the specimen.
• Focus on the specimen via the coarse/fine drive (3-10/5).
☞
Darkfield microscopy requires specimens and optical surfaces to be extremely clean. Finger
prints and traces of grease in particular have negative effects, since they brighten the
background of the field of view and reduce the image contrast.
Fig. 3-10 Setting of reflected-light darkfield
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