abi 7500 - Page 104

Chapter 5 Analyze the Relative Standard Curve Experiment
Analyze the Experiment
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
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Notes
Guidelines
When you analyze your own relative standard curve experiment:
• Immediately after a run, the 7500 software automatically analyzes the data using the
default analysis settings, then displays the Amplification Plot screen on your computer.
• To reanalyze the data, select all the wells in the plate layout, then click Analyze.
Navigation Tips
How to Select Wells
To display specific wells in the analysis screens, select the wells in the View Plate Layout tab:
1. To select wells of a specific type in the Select Wells With drop-down list, select
Sample, Target, or Ta sk, then select the sample, target, or task name.
2. To select a single well, click the well in the plate layout.
3. To select multiple wells: Click-drag over the desired wells, or CTRL+click or
Shift+click the desired wells in the plate layout.
4. To select all 96 wells, click the upper left corner of the plate layout.
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