abi 7500 - Page 192

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Chapter 9 Analyze the Comparative C

Experiment

View the Amplification Plot

Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve

and Comparative C

Experiments

168

Notes

4. View the baseline values (Plot Settings tab):

a. In the Graph Type drop-down list, select Linear.

b. Select the Baseline Start check box to show the start cycle and end cycle.

c. Verify that the baseline is set correctly: The end cycle should be set a few

cycles before the cycle number where significant florescence is detected. In the

example experiment, the baseline is set correctly.

Note: By default, the Amplification Plot displays the data for all targets present in

the experiment. Consequently, the plot overlays the baseline and threshold labels for

all targets until you select an individual target from the Target drop-down list.