Index
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
232
Q
QC Summary screen 103, 179, 201
QuickStart 13
, 206
R
radioactive waste, handling xix
ramp speed 24
, 25, 116, 117
Raw Data Plot screen 109
, 185
reaction mix
calculated volumes 37
, 39, 128
volumes 58
, 144
reaction plate
layout 14
, 15, 46, 134
load 68
prepare 60
, 146
standard versus Fast 6
unload from the instrument 74
Reaction Setup screen 36
, 127
reagents
other fluorescent-based 12
SYBR Green 11
TaqMan 11
reference sample
component of experiment 7
, 8
Relative Quantitation Settings screen 34
, 124
Relative Quantitation Settings screen 34
, 124
relative standard curve example experiment
analyze 76
description 14
design 20
name 22
prepare 50
workflow 17
, 18
relative standard curve experiments
about 7
compared to comparative CT experiments 9
repetitive motion, safety xxi
replicate, component of experiment 7
, 8
results, interpreting 77
, 157
RQ column 95
, 164, 198
RQ Max column 95
, 164, 198
RQ Min column 95
, 164, 198
run experiment
alerts 74
enable notification settings 69
for more information 74
guidelines 71
monitor 72
prepare for 67
, 153
start 71
workflow 66
Run Method library 36
, 126
Run Method screen 35
, 125
monitor during a run 73
S
safety
before operating the instrument xvi
biological hazards xxi
chemical xvii
chemical waste xix
conventions xii
electrical xx
ergonomic xxi
guidelines xviii
, xix
instrument operation xvi
moving and lifting instrument xvi
moving parts xx
moving/lifting xvi
physical hazard xx
repetitive motion xxi
standards xxii
workstation xxi
safety labels, on instruments xiv
safety standards xxii
safety symbols, on instruments xiii
Sample column 95
, 164, 198
sample dilutions
calculated volumes 40
, 53, 129, 142
prepare 53
, 142
Sample screen 31
, 121
samples
component of experiment 7
, 8
design guidelines 32
, 122
dilutions 53
, 142
prepare template 51
, 139
sample reactions (unknowns) 62
, 147
set up 31
, 121
select wells 80
, 160
settings, compare 202
singleplex PCR 10
, 26, 118
SPIKE flag 104
, 180
standard deviation, effect of threshold on 91
, 172
standard dilution series
calculated volumes 38
, 39
component of experiment 7
prepare 55
standard reaction plates, about 6
standard vs. fast plates 6
standards
component of experiment 7
design guidelines 30
diluting 55
EMC xxii
safety xxii
set up 29
Set Up Standards check box 26
, 28
standard reactions 62
Standards screen 29
study
about 188
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