abi 7500 - Page 257

Index
233
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
analyze 195
create 189
design 189
, 190, 192
properties 190
workflow 18
, 156
Study Properties screen 190
SYBR Green reagents 3
, 11, 25, 26, 28, 42, 110, 117,
118, 120, 130
symbols, safety xiii
T
TaqMan reagents 3, 11, 25, 26, 28, 42, 110, 117, 118,
120, 130
Target column 95
, 164, 198
targets
design guidelines 28
, 120
set up 26
, 119
Targets screen 26
, 119
Technical Support, contacting xi
Template 13
, 208
template. See samples.
text conventions viii
thermal cycling parameters
for plates added to a study 191
THOLDFAIL flag 104
, 180
threshold
correct values 90
, 171
examples 91
, 172
manually adjust 101
, 177
training, information on xi
troubleshooting
adjust baseline 101
, 177
adjust threshold 101
, 177
flags 104
, 180
omit wells 105
, 181, 199, 200
view analysis settings 100
, 176, 202
view Multicomponent Plot screen 107, 182, 200
view QC Summary screen 103
, 179, 201
view Raw Data Plot screen 109
, 185
U
unload instrument 74
user attention words, described viii
using this guide
as a tutorial 12
with your own experiments 13
W
WARNING, description xii
waste disposal, guidelines xix
Well Table 93
, 162
wells
negative control 32
, 47, 60, 122, 135, 146
omit 105
, 181, 199, 200
selecting 80
, 160
standard 32
, 47, 60, 122, 135, 146
unknown 32
, 47, 60, 122, 135, 146
workflows
Advanced Setup 204
example experiment 17
, 18
Export/Import 13
, 210
QuickStart 13
, 206
Template 13
, 208
workstation safety xxi