16 Agilent 2200 TapeStation System - User Manual
2 Specifications
ScreenTape Specifications
Specification (D5000 ScreenTape Assay)
Analytical Specifications D5000 ScreenTape assay and reagents
Sizing Range 100 – 5000 bp
Typical Resolution 400 – 5000 bp: 15 %
Sensitivity
1
1
signal-to-noise >3 (single peak)
0.1 ng/µL
Sizing Precision
2
2
Determined using the D5000 Ladder as sample
5% CV
Sizing Accuracy
2,3
3
Accuracy for run using single ScreenTape device
±10 %
Quantification Precision 0.1 – 1 ng/µL: 15 % CV
1 – 50 ng/µL: 10 % CV
Quantification Accuracy
4
4
Measured against 2100 Bioanalyzer system
±20 %
Quantification Range 0.1 – 50 ng/µL
Maximum Sample Buffer Strength 250 mM KCl,
250 mM Tris-HCl,
125 mM NaCl,
50 mM Acetate,
25 mM MgCl
2
,
25 mM BSA,
25 mM Guanidine-HCl
Physical Specifications
Multiple ScreenTape (96 well)
compatibility
Not compatible
Analysis Time 16 samples: <25 min
Samples per consumable 1 ladder + 15 samples
Sample volume required 1 µL
Kit stability 4 months
Kit size 112 samples/box
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