Agilent 2200 TapeStation System - User Manual 17
Specifications
2
ScreenTape Specifications
Specification (HS D5000 ScreenTape Assay)
Analytical Specifications D5000 ScreenTape assay and reagents
Sizing Range 100 – 5000 bp
Typical Resolution 400 – 5000 bp: 15 %
Sensitivity
1
1
signal-to-noise >3 (single peak)
5pg/µL
Sizing Precision
2
2
Determined using the High Sensitivity D5000 Ladder as sample
10 % CV
Sizing Accuracy
3
3
3
Accuracy for run using single ScreenTape device
±15 %
Quantification Precision 15 % CV
Quantification Accuracy
4
4
Measured against 2100 Bioanalyzer system
±25 %
Quantification Range 10 – 1000 pg/µL
Maximum Sample Buffer Strength 25 mM KCl,
25 mM Tris-HCl,
12.5 mM NaCl,
5 mM Acetate,
2.5 mM MgCl
2
,
2.5 mM BSA,
2.5 mM Guanidine-HCl
Physical Specifications
Multiple ScreenTape (96 well)
compatibility
Not compatible
Analysis Time 16 samples: <25 min
Samples per consumable 1 ladder + 15 samples
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples/box
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