5 Other Setup Tasks
To confirm the collision cell gas flow rate (6470A and 6495A/B only)
98 Agilent 6400 Series Triple Quad LC/MS System Installation Guide
i In the MS 2 tab, verify that the Prefilter DC 2 value is now appropriately
set.
Figure 49 MS2 tab with updated Prefilter DC 2 value
j Save the tune as atunes-MS2PrefilterFixed.TUNE.XML.
k Load this new tune file in the MRM method file and save the method.
8 Check that the tune abundance, mass axis, and peak width for all tune mass
ions meet requirements shown in Table 9 and Table 10.
Tabl e 9 Minimum recommended tune abundances at gain = 10
6
in POSITIVE Mode
(HED = -10 kV)
6495A/B 6470A
m/z MS1 MS2 MS1 MS2
118 4.E+06 2.E+06 4.E+05 3.E+05
322 2.E+06 1.E+06 2.E+05 1.E+05
622 2.E+06 1.E+06 2.E+05 1.E+05
922 8.E+05 4.E+05 2.E+05 1.E+05
1522 2.E+05 2.E+05 6.E+04 5.E+04
2122 5.E+04 6.E+04 3.E+04 4.E+04
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