68 Agilent 1260 Infinity VWD User Manual
4 Using the Detector
Special Settings of the Detector
Scanning with the VWD
1 Set up a run.
2 Start a run.
3 While running on the baseline, select from the menu Instrument >
More VWD > Blank Scan.
A background scan is stored in the memory.
4 When the peak of interest enters the flow cell, stop the flow (set flow
rate to zero or open the purge valve) and wait a few moments to
stabilize the concentration.
5 Select from the menu Instrument > More VWD > Sample Scan.
A sample scan is taken in the range defined under “Special
Setpoints” on page 70 and the Online Spectra window (see “Online
Spectra” on page 67) displays the result (Sample Scan minus Blank
Scan).
• Step 1: Blank Scan: scan of the background (solvent)
is stored in the memory.
• Step 2: Sample Scan: scan of the peak of interest is
taken while the peak stays in the flow cell (stop-flow
condition).
• Online Spectrum: Sample Scan minus Blank Scan.
Turning off the pump would stop the run and no access to the sample scan is possible.
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