Agilent 1260 Infinity VWD User Manual 77
How to optimize the detector
5
Match the Flow Cell to the Column
Figure 31 Influence of Cell Path Length on Signal Height
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the Agilent 1200 Series Infinity Variable Wavelength Detector
it is necessary to have more precise information on path lengths of the
VWD flow cells.
The correct response is:
expected response * correction factor
Please find below the details of the Agilent 1200 Infinity Series Variable
Wavelength Detector flow cells:
6WhdgWVcXZ
I^bZb^c
6cVanh^hd[eZhi^X^YZhiVcYVgY
&%"bbdei^XVaeVi]aZc\i]
+"bbdei^XVaeVi]aZc\i]
Tabl e 11 Correction factors for Agilent VWD flow cells
Part number Path length (actual) Correction factor
Standard flow cell 10 mm, 14 µL, 40 bar (G1314-60186) 10.15 ± 0.19 mm 10/10.15
Semi-micro flow cell 6 mm, 5 µL (G1314-60183) 6.10 ± 0.19 mm 6/6.10
Micro flow cell 3 mm, 2 µL, 120 bar (G1314-60187) 2.80 ± 0.19 mm 3/2.8
High pressure flow cell 10 mm, 14 µL, 400 bar (G1314-60182) 10.00 ± 0.19 mm 10/10
However you have to be aware that there are additional tolerance of gasket thickness and
its compression ratio which is supposed to be very small in comparison with the machining
tolerance.
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