30 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Setting up a Fraction Collector Method
Whenever the Peak Based mode is specified fractions will be collected based on
the peak detection parameters given in the Peak Detector table. Additionally a
Maximum Peak Duration in minutes has to be specified. This parameter is
mandatory if you use Peak Controlled fraction collection, but is disabled for
Time Based fraction collection.
When the Time Based mode is chosen two different options are available:
• The # of Fractions can be edited to collect a fixed number of equal fractions
in a give time interval. This time interval is defined by the time value in the
current and following timetable line.
• Timeslices [min] can be edited to collect fractions with a defined collection
time. With this option the collection time of the last fraction can be shorter.
This depends on the overall runtime.
For editing the Timetable the functions Insert, Append, Cut, Copy and Paste are
offered.
To access the additional sections in the Setup Fraction Collector dialog box
click the More button.
Time
In the time section of the dialog box the Stoptime and the Posttime for the
fraction collector can be specified. By default the Stoptime is set to as pump
and the posttime is switched off.
Rinse Fraction Collection Needle (Analytical Scale only)
If Discrete Fractions is selected as Collection Mode (see also“Collection
Mode” on page 12), you can setup a needle rinse step before the fraction
collection and/or between fractions. Then the needle will move to the funnel
on the internal tray and the diverter valve will switch to flush the needle in
order to avoid carry over from the previous fraction. The instrument will
determine, if it is possible to rinse the needle before the next fraction is
expected.
If you have recovery positions in your fraction collector or if you are using one
fraction collector for sample recovery in a multiple fraction collector
configuration, the rinse function between fraction collection is ignored.
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