3Using the Module
Setting up the Pump with the Instrument Control Interface
1260 Infinity II Flexible Pump User Manual 78
Table 9 Method parameters
Parameter Limits Description
Flow 0.00 – 5.00 mL/min in
steps of 0.001.
Recommended flow
range:
0.05 – 5.00 mL/min.
The flow is the rate of movement of eluent along the column. It is important
that the flow rate is kept constant to ensure precise retention time, and peak
measurements. Variations in flow rate can occur as a result of the partial
failure of the pumping system, air in the pumping system, a change in the
mobile phase viscosity or a temperature change.
Enable Blend Assist Mark this check box to switch on Blend Assist, which allows you to set up
solvent mixtures from stock solutions. When this check box is marked, the
Blend Assist section of the method setup is available.
Solvents Blend Assist Disabled: When Blend Assist is disabled, you can set the
percentages of solvents B, C and D to any value from 0 through 100 %.
Solvent A always delivers the remaining volume: 100 - (%B + %C + %D). The
check boxes allows you to turn the solvent channels on (checked) or off
(cleared). Click the solvent name down arrow and select the solvent from
the list of calibrated solvents and solvent mixtures. For solvent mixtures,
specify the percentage of additive. You can enter your own name for the
solvent or solvent mixture in the adjacent field.
Blend Assist Enabled: When Blend Assist is enabled, the table shows the
solvent blends that have been set up in the Blend Assist section of the
method setup.
•Solvent: The solvent or blend of solvents as set up in the Blend Assist
section.
•Used: Mark this check box if you want to use this solvent or blend in the
method.
•%: Enter the percentage of the solvent or blend in this field.
•Name: Type a name for the solvent or blend in this field.
Stoptime 0.01 – 99999 min or
As Injector/No Limit
(an infinite run time).
The stoptime sets a time limit for your analysis. After the stoptime, all
gradients are stopped and the pump parameters return to their initial values.
The pump can be used as a stoptime master for the complete analytical
system. The pump also stops the detectors if they have a No Limit stoptime
setting. If no limit is given, a method will have to be stopped manually.
Posttime 0.01 – 99999 min or
Off (0.0 min).
Your instrument remains in a not ready state during the posttime to delay
the start of the next analysis. You can use the Posttime to allow your column
to equilibrate after changes in solvent composition (for example after
gradient elution).
To Next Page
Loading...
Need help?
General
