PCR Programs 4
To manually create programs
Agilent SureCycler 8800 Setup and User’s Guide 37
Use the following steps to manually generate a new PCR program.
1 From the Home screen, press New Pr
ogram. The programing screen
opens.
2 From the available program steps listed in the Command List on t
he
left side of the screen, determine the order of steps that will make up
the new PCR program.
See “Program Steps in the Commands List” on page 43.
3 A
dd the first step of the program from the Command List
to the
Program Window using one of the following two approaches:
• Press the command then press the forward arrow button (shown
below).
• Double- press the command.
Both approaches open a new screen in which you can enter the
parameters fo
r the selected step.
4 Enter the necessary settings for the newly added step as prompted.
5 Confirm your settings by pressing OK. Y
ou are re
turned to the
programming screen.
6 Repeat step 3 through step 5 for each program step to be added to the
program. You can change the or
der of commands in the Command
Window at any time using the up/down arrow buttons (shown below).