Appendix A: General Sampling with the P100
PURPOSE
To describe the procedure to monitor for viable airborne bacteria with the P100 Portable Microbial Air Sampler
from EMTEK, LLC.
A.1 P100 Operating Principles
A 90 mm agar based test plate is placed on the adjustable stage of the P100 underneath the inlet cover assembly.
The required plate height is set with the aid of the adjustable stage and media distance indicator on the inside top
of the inlet lid. The required sample volume or time period is set on the P100 set up screen and the testing period
is initiated. During testing, the vacuum source of the P100 draws 28.3 LPM (1 CFM), or 100 LPM into the P100
through the sample inlet. Airborne bacteria in the sampled volume of air become impinged (captured) on the test
plate. The sampled air volume is then drawn through CPU controlled flow system. The sampled air volume then
passes through a 0.2 micron HEPA filter, as is exhausted at the rear of the unit. An additional option allows for
attachment of remote exhaust tubing to move the exhaust well away from the test location. Upon completion of
the test period, the test plate is removed from the P100 and is incubated for a designated time period at a specified
temperature. Following the required incubation period, the number of bacterial Colony Forming Units (CFU)
are enumerated. The density of air borne bacteria per volume of air tested can then determined.
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