Appendix D: Suggested Sample Submission and Results
Recording
This section applies to all sampler types.
1. Submit the test plate(s) and test parameter information to the appropriate laboratory for recording and further testing.
2. Qualified personnel record the required test parameter information on the appropriate electronic or hard copy test report
form and place the test plates “On-Test” in incubation.
3. Incubate the test plates with an appropriate incubation scheme (e.g., 30-35°C for a minimum of 5-days [other incubation
requirements or schedules may be used as appropriate]).
4. Following the required incubation period, count all Colony Forming Units (CFU) found within the air impingement
zone.
5. Determine Total CFU/ Specified Volume and record the results in the appropriate section of the electronic or hard copy
test report form. This result is calculated as follows:
Total CFU per Test Plate = CFU/ Identified Volume (L/ft
3
/m
3
)
Volume Sampled
EXAMPLE:
If the test period was set to sample at 100 LPM for 10-minutes, the total volume collected would be 1000 Liters (L) or 1
cubic meter (m
3
). If following incubation, 20 CFU were recovered in the impingement zone of the plate. The
calculation would be:
• Total CFU/Test Plate = 20
• Total Volume = 1000 L (or 1 m
3
)
20 CFU/Test Plate = 20 CFU = 0.02 CFU/L (or 20 CFU/m
3
)
1000 L (or 1 m
3
) 1000 L (or 1 m
3
)
6. Record date “Off-Test” in the appropriate section of the test report form.
7. If Microbial Identification of organism recovered is required, submit plate for ID according to applicable procedures
related for sample submission and processing for Microbial Identification. Discard all other plates in an appropriate
Bio-Hazard container for disposal.
8. Test Report Forms, once completed, should be reviewed for accuracy and completeness, and signed by a second
qualified analyst.
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