ActionPossible causeProblem
Ensure that you use the volumes
specified in the software for the run.
Larger volumes may be needed if the
problem persists.
Insufficient volumes of
sample and/or reagents.
Air is injected
during sample
or reagent in-
jections.
Ensure that you use the recommended
caps for sample and reagent vials.
Evaporation from uncovered vials will
both affect the concentration and re-
duce the volume.
Perform a System Check and Pump
Calibration to calibrate the peristaltic
pump.
Always fill the water vial in the rack
before starting the run.
Not enough water to
clean the injection nee-
dle.
Samples be-
come cross-
contaminated.
Temperature problems
If the analysis temperature is unstable (the Temperature indicator flashes) for more
than one hour after the temperature has been set, check that:
•
there is at least 20 cm clearance around the instrument to allow adequate circulation.
•
the instrument is not placed adjacent to heaters, cooling ducts, or in direct sunlight.
Other instrument-related
problems
Unexpected detection results may appear if there are dust or other particles on the
sensor chip surface. Never remove the sensor chip from its protective cassette.
Biacore X100 Operating Instructions 28961142 AD 81
7 Troubleshooting
7.1 Instrument related problems
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