ActionFailed test
Check that the pump cover is properly closed.A: Peristaltic
pump calibration
Check that the buffer tubes reach the buffer liquid and that they
are not squeezed.
Check that the pump tube does not leak and has not been
punctured by mistake.
If the test fails repeatedly, change the pump tube.
If the Fc-values in the results are too low, check that you used
a non-immobilized Sensor Chip CM5.
B: Response
If the Fc-values in the result are too low or too high, check that
you used the correct buffer (HBS-EP+ buffer) and test solution
(BIAtest solution).
Check that the tubes do not leak.C: Injections
Contact your local GE service representative.
Check that you used a non-immobilized Sensor Chip CM5.D: Noise
Check that the temperature stability is not affected by inade-
quate ventilation, exposure to direct sunlight or similar.
Liquid flow problems
ActionPossible causeProblem
Ensure that there is enough buffer be-
fore starting the run.
There is not enough
buffer in the buffer bot-
tle.
Buffer is not
supplied proper-
ly.
Close the cover.The cover of the peri-
staltic pump is not prop-
erly closed.
Check for leaks (CheckLeaks).The flow system is leak-
ing.
80 Biacore X100 Operating Instructions 28961142 AD
7 Troubleshooting
7.1 Instrument related problems
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