MEDIAJET – Operating instructions V09 Operation
42 INTEGRA Biosciences AG
4.9 Mixing additives into the agar
The MEDIAJET, in connection with the peristaltic pump DOSE IT, can mix
continuously an additive in the concentration of 3 to 10 % into the medium. This
setup is typically used for the preparation of blood agar. Please also refer to the
DOSE IT operating instructions.
4.9.1 Installation
Place the DOSE IT pump close to the right side of the MEDIAJET. Plug one
side of the electrical interface cable into the socket marked with “Pump” on
the back of the MEDIAJET and the other side to the foot switch socket on the
back side of the DOSE IT pump.
4.9.2 MEDIAJET Settings
Press FILL DISHES in the MAIN MENU for adapting a dish filling program.
Select the desired program and press PROGRAM SETTINGS in order to
adjust it (see also “4.4
Adapting a dish filling program” on page 30).
You need this value for the DOSE IT program parameters (see below).
4.9.3 Determine the dosing volume of the additive
Determine the volume of additive to be added per dishes to achieve the desired
percent mixture.
For example, if the total dispensing volume in the Petri dish is 18 ml and the final
concentration of additive is 5 %, the dosing volume of the additive is 0,9 ml
(18 ml × 0.05). You need to enter this value in the DOSE IT program parameter
(see next page).
When connecting a sterilized Tubing set for the
preparation of blood agar, the small diameter
tubing (2 x 5 mm) has to be entered into
DOSE IT pump head and the big diameter
tubing (6 x 9 mm) into the MEDIAJET pump
head. The glass T-piece needs to be located
ahead of the MEDIAJET pump head.
Set the desired total Dispensing volume
(agar and additive) to be filled into the Petri
dishes.
Select Pump speed and press CHANGE to
get the ‘Estimated dosing time’ displayed,
automatically calculated by the MEDIAJET.
To Next Page
Loading...
