VIAFLO 96/384 – Operating instructions V11 Operation
24 INTEGRA Biosciences AG
4.3.4 Recommendations for pipetting
INTEGRA Biosciences recommends the following techniques for enhancing pipetting
results. These techniques are consistent with ISO standard 8655-2.
• It is best to immerse the GripTips just enough in liquid to allow the desired volume to be
aspirated.
• Always pre-wet GripTips. After loading tips onto your pipette, aspirate and dispense the
full volume 2-3 times to coat the inside of pipet tips. Pre-wetting ensures that the liquid
and air inside the tips are at equal temperature and the dead air space is humidified.
• VIAFLO 96/384 is an air displacement pipette. It requires to touch the GripTips against
the side of the well or dip them into the liquid after a dispense. This process is referred
to as “touching off” or “tip touch” and prevents liquid from clinging to the pipette tips.
• In programs such as Repeat Dispense, a first and last dispense can be programmed.
These two dispenses are not used and are dispensed into the waste as they contain the
accumulated pipetting errors. Using a first and last dispense is recommended if
accuracy and precision are of high importance.
• Viscous samples should be aspirated and dispensed at the slowest speeds to ensure
accurate pipetting. In addition, the pipetting mode “Reverse pipet” can be used to
optimize pipetting results with viscous samples.
• For pipetting liquids with high vapor pressures (such as methanol or ethanol), use
relatively fast pipetting speeds and avoid prolonged pauses after aspiration.
• Calibrate based on fluid type. VIAFLO 96/384 is tested and calibrated at the factory for
use with distilled water at room temperature. It may be necessary to re-calibrate your
VIAFLO 96/384 if the liquid to be used has different physical properties (specific gravity
and vapor pressure) than water. Calibration mode can be accessed in the Toolbox
menu.
W
ARNING
Avoid pipetting for extended periods. To minimize the risk of repetitive strain
injury, include regular pauses of several minutes.
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