Integra VIAFLO 96 - Sample Dilute Mode; Pipet;Mix Mode

VIAFLO 96/384 – Operating instructions V11 Pipetting modes
34 INTEGRA Biosciences AG
5.2.3 Sample dilute mode
Application: Accomplish accurate sample dilutions by using diluent to “chase” small
sample volumes from the pipet tips. An air gap keeps liquid separated in the tips and helps
to minimize carryover of diluent when aspirating the sample.
Operation:
• Press and release the RUN key to initiate each aspiration (remove tips from liquid for
air-gap aspiration).
• Press and hold the RUN key to perform a two-step blowout. The entire tip contents will
be dispensed together.
5.2.4 Pipet/mix mode
Application: Use this mode when mixing is required immediately after transfer of liquid.
This mode saves a programming step by incorporating the mix option after dispensing.
Operation:
• Press and release the RUN key to initiate the aspiration.
• Press and release the RUN key to dispense. Mixing occurs automatically after the
dispense step.
• Upon completing the desired number of mixes, a blowout is initiated automatically
prompting you to remove the tip(s) from the liquid and press the RUN key to complete
the blowout.
Options Steps Description
Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip.
Air Gap Sets the volume of the air gap to keep both liquids separated.
Aspirate 2 Sets the volume of the sample in the tip.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast).
Speed Sets speed of the current pipetting step.
Custom Converts the predefined program into a custom program.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is equal to the dispense
volume.
Mix Sets the mixing volume after dispensing.
Mix Cycles Sets the number of mix cycles.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Mix Speed Sets speed uniquely for mixing (1 = slow, 10 = fast).
Speed Sets speed of the current pipetting step.
Custom Converts the predefined program into a custom program.