Integra VIAFLO 96 - Repeat Dispense Mode

VIAFLO 96/384 – Operating instructions V11 Pipetting modes
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5.2.2 Repeat dispense mode
Application: This mode can be used for fast reagent addition to microplates from one
source container. You can dispense a large aspirated volume of liquid in multiple aliquots
to multiple targets.
Operation:
• With the tip(s) in liquid, press and release the RUN key to initiate the aspirate step.
• Press and release the RUN key for every dispense. Alternatively, press and hold Run
to execute paced dispenses. The dispense number is shown on the display.
• The pipette will stop paced dispenses when it reaches the Last Dispense. You can
choose to use this Last Dispense or discard it.
- If reuse of last dispense is not activated, press and hold the RUN key to purge the
Last Dispense volume with a two-step blowout.
- If reuse of last dispense is active, you can start the next repeat dispense cycle with
aspirating liquid to the last dispense in the tip. To finish the repeat dispense cycle,
press PURGE.
Options Steps Description
Edit Dispense Sets the volume for repetitive dispensing. The aspirated
volume is calculated automatically.
First
Dispense
A pre-dispense volume can be selected independently to
improve accuracy and precision. The dispense is discarded.
Last Dispense A last-dispense volume can be selected independently to
improve accuracy and precision. The dispense is discarded.
Reuse Last
Disp.
By default (red ), the mode ends with dispensing of the last
dispense. This aliquot contains the accumulated error from all
prior dispenses.
If you want to reuse the last dispense, press OK (green ).
At the end of the program the last dispense remains in the tip,
while the pipette is ready to aspirate a new volume to start the
next repeat dispense run.
Count The maximum number of dispenses possible (count) is
calculated automatically. This count may be reduced to the
desired number.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast).
Speed Sets speed of the current pipetting step.
Pace Sets the time duration between dispenses in repeat pipetting,
if keeping RUN key pressed (1 = long, 9 = short).
Custom Converts the predefined program into a custom program.