VIAFLO 96/384 – Operating instructions V11 Pipetting modes
36 INTEGRA Biosciences AG
5.2.6 Reverse pipet mode
Application: With this mode the aspiration volume is higher than the volume dispensed.
It is recommended for liquid transfers of viscous and high vapor pressure liquids. The
dispense method prevents introduction of air into the sample because no blowout is made.
Operation:
• Press and release the RUN key to initiate the aspiration. The total volume aspirated is
the sum of desired dispense volume and last dispense volume.
• Press and release the RUN key to dispense the programmed volume.
• If reuse of last dispense is not activated, press and hold the RUN key to purge the Last
Dispense volume with a two-step blowout.
• If reuse of last dispense is active, you can start the next reverse pipet cycle with
aspirating liquid to the last dispense in the tip. To finish the reverse pipet cycle, press
PURGE.
Options Steps Description
Edit Dispense Sets the dispense volume.
Last Dispense Sets the volume to leave in the tip until final blowout.
Reuse Last
Disp.
By (red ) default, the mode ends with dispensing of the last
dispense.
If you want to reuse the last dispense, press OK (green ).
At the end of the program the last dispense remains in the tip,
while the pipette is ready to aspirate a new volume to start the
next reverse pipet run.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast).
Speed Sets speed of the current pipetting step.
Custom Converts the predefined program into a custom program.
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