VIAFLO 96/384 – Operating instructions V11 Pipetting modes
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5.2.7 Variable dispense mode
Application: Use this mode when differing dispense volumes are required. This mode
could be used to quickly set up a dilution series in plates or for feeding similar samples to
different assay plates where different sample volumes are needed.
Operation:
• Press and release the RUN key to initiate the aspiration of total volume.
• Press and release the RUN key to initiate each subsequent dispense. The pipette stops
and beeps when ready for the Last Dispense step, i.e. to purge the calculated waste
volume amount.
• Alternatively, press and hold the RUN key to execute paced dispenses. The pipette
stops paced dispensing when it reaches the Last Dispense. This aliquot contains the
accumulated error from all prior dispenses. You can choose to use this Last Dispense
or discard it.
• During the Last dispense, press and hold the RUN key to perform a two-step blowout.
Options Steps Description
Edit Count Sets the total number of dispensing steps.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast).
Dispense
1...Count
Sets different volumes for repeated dispensing. Number of
dispenses and dispense volume cannot exceed nominal
volume of the pipetting head. The total volume is
automatically calculated.
Speed Sets speed of the current pipetting step.
Pace Sets the time interval between dispenses, if keeping RUN
key pressed (1 = long, 9 = short).
Custom Converts the predefined program into a custom program.
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