Integra VIAFLO 96 - Serial Dilution Mode

VIAFLO 96/384 – Operating instructions V11 Pipetting modes
40 INTEGRA Biosciences AG
5.2.10 Serial dilution mode
Application: Use this mode to perform serial dilutions. The Serial Dilution mode enables
aspiration of a specific volume followed by a mix sequence and ending with the original
aspiration volume in the tips.
N
OTE
For serial dilutions it is helpful to switch Column Detent on, see “Tip Align”
under "
Position Settings" on page 16.
Operation:
• Attach 8/16 GripTips on the leftmost row by lowering the Pipetting unit onto the tips.
CAUTION
When prompted to push the Tip load button, first press OK (14) to switch to
“low power” loading mode. Then press the Tip load button and at the same
time push down the Control unit to load the tips with reduced forces.
• Replace the tip box with the microplate and put the reagent container on the other
position, e. g. position A. Press and release the RUN key to initiate the aspiration of the
reagent.
• Press and release the RUN key to start the dispense and mix sequence. Proceed with
the next rows.
• Rows (first number) and Mix Cycles (second number) are tracked on the display. Mix
Cycles are shown in red when mixing. A green dot on the column number indicates the
active program step.
Options Steps Description
Edit Aspirate Sets the aspiration volume that is identical to the dispense
volume.
Mix Sets the mixing volume after dispensing.
Mix Cycles Sets the number of mix cycles.
Rows Sets the number of rows. A row indicator will notify the
number of dilutions performed.
Asp. Speed Sets speed uniquely for aspirating (1 = slow, 10 = fast).
Mix Speed Sets speed uniquely for mixing (1 = slow, 10 = fast).
Disp. Speed Sets speed uniquely for dispensing (1 = slow, 10 = fast).
Speed Sets speed of the current pipetting step.
Custom Converts the predefined program into a custom program.