VIAFLO 96/384 – Operating instructions V11 Pipetting modes
46 INTEGRA Biosciences AG
Example of automatic custom program
The task is to perform a serial dilution over the entire 96 well plate. The concentrated
sample is in column 1. The custom program would be set up as followed (note that position
values might slightly differ when adapting the protocol on a different unit):
N
OTE
GripTips cannot be attached automatically.
5.4 VIALINK
VIALINK is a pipette management software for the PC and is used to manage VIAFLO 96/
384 and VIAFLO electronic hand held pipettes. It allows to create custom programs,
manage a library of custom programs, establish a service history and more.
To establish a connection between computer and VIAFLO 96/384, a standard USB cable
(type A to B) is required.
The software VIALINK can be downloaded from the INTEGRA website in the product
section and is free of charge for all VIAFLO 96/384 customers. A detailed description of
the software, along with the operating instructions, can be found on the website as well.
Program step Action
1) Move X,Z: X -16.6 mm, Z 30,1 mm Sets the start position to aspirate the sample
in the first column.
2) Aspirate liquid: 100 µl The concentrated sample is aspirated.
3) Mix: 2x 100 µl The sample is re-suspended during mixing.
The aspiration volume of 100 µl remains in
the tips after mixing.
4) Move Z: 40.8 mm The pipetting head moves upwards.
5) Move X: 9.0 mm The pipetting head moves to the next column
on the right.
6) Move Z: 30.0 mm The pipetting head moves down into the
well.
7) Mix 3x: 150 µl During mixing the sample is diluted, 100 µl
remain in the tips after mixing.
8) Loop: Step 5, Count 11 Continue serial dilution in the next wells
(repeats steps 5-8 eleven times).
9) Move Z: 80.7 mm The pipetting head moves upwards.
10) Move X,Z: X -75.8 mm, Z 52.6 mm The pipetting head moves to the waste
container.
11) Purge: Speed 8 The residual liquid is dispensed into the
waste container.
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