Integra VIAFLO 96 - Pipetting Modes; Overview Pipetting Modes

VIAFLO 96/384 – Operating instructions V11 Pipetting modes
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5 Pipetting modes
This chapter describes how to program the VIAFLO 96/384 in two ways:
• Function-based pipetting modes: You can select from ten predefined pipetting modes
that you can quickly and easily edit and execute. They are described in the
following sections.
• Custom step-based programming mode: You can create and store up to forty multi-
stepped pipetting protocols on the pipette using the basic functions of
“Aspirate, Dispense, Mix, Purge, Prompt, Set Z Height, Tip Align and Loop” presented
in “5.2
Detailed description of pipetting modes” on page 32. The custom programming
mode is described in “5.3
Custom step-based programming mode” on page 41
5.1 Overview pipetting modes
The table below provides an overview of the selectable pipetting modes. All modes are
accessed from the Main Menu. Use the Touch wheel to scroll to your desired pipetting
mode.
Press the OK to access the pipetting mode and to start defining parameters. Press  to
select the Help option.
Pipetting mode Description
Pipet Allows liquid transfers when aspirate and dispense volumes are
equal.
Repeat Dispense Allows dispensing multiple aliquots of the same volume without
refilling the tips after each dispense for fast microplate filling and
processing.
Sample Dilute Allows aspirating of sample and diluent divided by a defined air
gap into one tip, followed by a complete dispense.
Pipet/Mix Transfers a defined volume and follows with a defined number of
automatic mixing cycles.
Manual Pipet Allows the operator to manually control the aspiration and
dispensing up to the set volume.
Reverse Pipet Allows liquid transfers of viscous or high vapor pressure liquids
by preventing introduction of any air into the sample. The
aspiration volume is higher than the volume to be dispensed.
Variable Dispense Allows dispensing multiple aliquots of different volumes.
Variable Aspirate Allows aspirating multiple aliquots of different volumes.
Sample Dilute/Mix Allows aspirating two liquids separated by an air gap followed by
a complete dispense and Mix step.
Serial Dilution Allows aspirating a transfer volume followed by a mix. Rows and
Mix Cycles are tracked on the display.
Custom Allows to create and store of up to 40 multi-stepped pipetting
protocols.