VIAFLO 96/384 – Operating instructions V11 Pipetting modes
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Example of manual custom program
The task is to combine 2 different liquids in a 96 well plate for a kinetic assay and then
mixing it to achieve a homogeneous solution. The microplate should then be incubated for
5 minutes and the content is then distributed to a 384 well plate. The custom program
would be set up as followed:
N
OTE
The program steps 8-11 can be shortened with the Loop step:
9) Loop to step 8, 4 times
10) Purge
Program step Action
1) Aspirate liquid 1: 160 µl (e.g. diluent) With tips in liquid 1 press RUN key.
2) Aspirate air: 20 µl Move tips out of liquid and press RUN key.
3) Aspirate liquid 2: 50 µl (e.g. reagent) With the tips in liquid 2 press RUN key.
4) Dispense: 230 µl Press and hold RUN key until liquid is
dispensed and tips are removed from the
liquid (two-step blowout).
5) Mix 3x: 200 µl Press RUN key.
6) Prompt: Incubate 5 min No action.
7) Aspirate: 210 µl With tips in the 96 well plate, press RUN key.
8) Dispense: 50 µl Move the Plate slider to the front and press
RUN key to dispense the back positions of
the 384 well plate.
9) Dispense: 50 µl Move the pipetting head one well to the right
and press RUN key to dispense the second
columns.
10) Dispense: 50 µl Move the Plate slider to the back and press
RUN key to dispense the front positions of
the 384 well plate.
11) Dispense: 50 µl Move the pipetting head to the left and press
RUN key to dispense the first columns.
12) Purge Purge (does not need to be programmed).
The residual liquid is dispensed into the
waste container. Press and hold RUN key
until liquid is purged and tips are removed
from the liquid (two-step blowout), see “4.3.3
Blowout modes” on page 23.
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