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Leica DM2000 - Start-up: Phase Contrast Setup; Phase Contrast Ring Centering

Leica DM2000
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33
7. Start-up
7.3 Checking Phase Contrast Rings
If your microscope is equipped for the use of
phase contrast, the light rings that fit the objec-
tives are built into the condenser.
The light rings are already centered in the
factory. However, the centering should be
rechecked.
Note:
A light ring slide which is inserted into the side
of the condenser is used for condensers without
condenser disks. Centering is not required in
this case.
Note:
When swiveling in a suitable objective for
phase contrast, the corresponding light ring
must be chosen.
The objective engraving (e.g. PH 1) indicates the
corresponding light ring (e.g. 1).
In the place of an eyepiece, insert the focus-
ing telescope (Fig. 36) into the observation
tube.
•Swivel in the phase contrast objective with
the lowest magnification.
Focus on the specimen with the focus wheel.
Focus the ring structure (37.a) by slightly loos-
ening the clamping ring (36.2) and moving the
eye lens (36.1).
Retighten the clamping ring.
Select the corresponding ring diaphragm
(light ring) in the condenser.
If the light ring and the phase ring are not
shown as arranged in Fig. 37.c, the light ring
must be centered.
Fig. 36 Focusing Telescope
1 Adjustable eye lens
2 Clamping ring for fixing the focus position
1
2
PH LR
Fig. 37 Phase Contrast Centering Procedure
PH=phase contrast ring, LR=light ring
a Condenser in brightfield (BF) position
b Condenser in phase contrast (PH) position
Light ring (LR) not centered
c Light ring and phase ring centered
ab c

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