Lonza PyroTec PRO - Air Liha Multisense Functions

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PyroTec™ PRO Hardw are User’s Manual, V1.2 41
RT-MN024-1.2 02/21
During dispense, the spindle rotates in reverse direction, the plunger moves down and
presses the liquid out of the tip.
Air Chamber
In the lowest position, the plunger end is close to the disposable tip to keep the dead vol-
ume in the air chamber low.
The sealing between the plunger and the air chamber seals the chamber gas-tight against
ambient air.
The controlled variation of the volume in the air chamber enables precise aspirate and dis-
pense actions. A sophisticated electronic motor control moves the plunger accordingly.
Tip Adapter
The pressure sensor and electronics in the tip adapter are used for the Air LiHa MultiSense
functions.
To prevent inner contamination by sample liquid, a replaceable inline filter is enclosed in
the tip adapter. In case of an erroneous aspiration action, e.g. if a wrong disposable tip is
mounted and, as a result, too much liquid is aspirated, the liquid reaches the filter. This ef-
fects a pressure change in the air chamber and the pressure sensor causes the plunger
movement to stop.
If the inline filter got contact with sample liquid, it needs to be replaced.
DiTi Eject
Device
The lower DiTi eject device allows to eject disposable tips at a low position. Together with
the optional DiTi waste cover and the DiTi waste slide, it prevents from spilling sample liq-
uid onto the worktable, minimizes spreading of aerosols and thus minimizes contamina-
tion risks.
5.3.2 Air LiHa MultiSense Functions
Purpose of the
MultiSense
Functions
The Air LiHa MultiSense functions are used for pipetting tasks with Air LiHa and disposable
tips (DiTis). They include:
• cLLD (capacitive liquid level detection)
• pLLD (pressure based liquid level detection)
• PMP (pressure monitored pipetting)
Capacitive
Liquid Level
Detection
The cLLD function measures the capacitance between the tip and the carrier containing
the labware with the sample. As soon as the tip touches the liquid surface, the change in
capacitance triggers a detection signal. cLLD records the height of the tip at the time of the
trigger signal. This only works with conductive liquids and conductive DiTis.
This function is not limited to the Air LiHa MultiSense functions. It is the same with the
standard tip adapters.