Operating Your Analyzer
6-5
6.5 Sample Collection and Handling
z Samples, controls, calibrators and waste are potentially infectious. Wear
proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow
safe laboratory procedures when handling them in the laboratory.
z Avoid direct contact with blood samples.
z Do not re-use disposable products.
z Be sure to use clean K
2
EDTA anticoagulant collection tubes, fused silica
glass/plastic test tubes and 20µL borosilicate
glass capillary tubes.
6.5.1 Whole Blood Samples
Collect and handle the whole blood sample as follows:
1. Collect venous blood with a K
2
EDTA(1.5 - 2.2mg/ml)anticoagulant collection tube;
2. Rapidly and thoroughly mix the blood with the anticoagulant;
z Be sure to use clean K
2
EDTA anticoagulant collection tubes, fused silica
glass/plastic test tubes and 20µL borosilicate
glass capillary tubes.
z For the whole blood samples to be used for WBC differential or PLT count,
you shall store them at the room temperature and run them within 8 hours
after collection.
z If you do not need the PLT, MCV and WBC differential results, you can store
the samples in a refrigerator (2℃ - 8℃) for 24 hours. You need to warm the
refrigerated samples at room temperature for at least 30 minutes before
running them.
z Be sure to mix any sample that has been prepared for a while before running
it.