Understanding the System Principles
3-3
3.3 Dilution
Usually in blood samples, the cells are too close to each other to be identified or counted. For
this reason, the diluent is used to separate the cells so that they are drawn through the
aperture one at a time as well as to create a conductive environment for cell counting.
Moreover, red blood cells usually outnumber white blood cells by 1,000 times. For this reason,
lyse needs to be added to the sample to eliminate the red cells before the WBC counting.
When analyzing a whole blood sample, this analyzer aspirates 13µL of the sample and
follows the procedure presented in Figure3-1 to dilute it before proceeding to the actual
analysis.
Figure3-1 How a whole blood sample is diluted
About 1:269 dilution
15.6uL
0.5mL of lyse
About 1:308 dilution for
the WBC/HGB analysis
About 1:44872 dilution for
the RBC/PLT analysis
About 2.6mL of diluent
13uL of Whole blood sample
3.5mL of diluent
To Next Page
Loading...
