Using the Calibration Programs
9-3
9.3 How to Calibrate
The analyzer provides 3 calibration programs: manual calibration, auto calibration using
commercial calibrators and auto calibration using fresh blood samples. Two sets of
calibration factors are prepared respectively for the whole blood mode and the predilute
mode.
9.3.1 Preparing Your Analyzer
Do the following pre-calibration procedures before calibration. If problems are detected
during these checks, do not attempt to calibrate the analyzer. If necessary, call Mindray
customer service department or your local distributor for assistance.
Check and make sure enough reagents have been prepared for the calibration. You need to
start over the calibration if the reagents run out during the process.
Do the background check. If the analyzer alarms for abnormal background results, see
Chapter 11 Troubleshooting Your Analyzer for solutions.
Enter the “Count” screen and run a vial of normal control 11 consecutive times. Enter the
“Review” screen to check the reproducibility of the second to eleventh runs and make sure
they meet the following requirements.
Table 9-1 Reproducibility
Parameter Expected range CV%
WBC
7.0 - 15.0 × 10
9
/ L
≤ 2.0
RBC
3.50 - 6.00 × 10
12
/ L
≤ 1.5
HGB 110 - 180 g/L ≤ 1.5
MCV 80.0 - 110.0 fL ≤ 0.5
PLT
150 - 500 × 10
9
/ L
≤ 4.0
At the “Count” screen, run a vial of high control three consective times and then immediately
run the diluent three consective times, calculate the carryover per the following equation.
％
－
－
100
result sample level-low hirdTresult sample level-high hirdT
result sample level-low hirdTresult sample level-lowFirst
(%)Carryover ×=
The calculated carryovers shall meet the following requirements: WBC, RBC and HGB
shall be no greater than 0.5 % ; PLT shall be no greater than 1%.
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