Understanding the System Principles
3-7
(readings taken when there is only diluent in the bath). The HGB is calculated per the
following equation and expressed in g/L.
HGB(g/L) = Constant×Log
10
(Blank Photocurrent/Sample Photocurrent)
3.4.3 Derivation of WBC-Related Parameters
WBC
WBC (10
9
/ L) is the number of leukocytes measured directly by counting the white blood cells
passing through the aperture. Note that when you observe NRBCs (nucleated red blood
cells), which do not react with the lyse and can be mistaken by the analyzer for white cells, in
the microscope, be sure to correct the system-generated result by the following formula,
where WBC represents the system-generated white cell number, NRBC the number of
NRBCs counted in 100 white cells and WBC′ the corrected white cell number.
WBC differentia
With the help of the diluent and lyse, this analyzer can size the white cells into three
sub-populations - lymphocytes, mid-sized cells (including monocytes, basophils and
eosinophils ) and granulocytes. Based on the WBC histogram, this analyzer calculates
Lymph％, Mid％ and Gran％ as follows and express the results in percents.
where PLT = particles in the lymphocyte region(
L/10
9
)
PM = particles in the mid size region(
L/10
9
)
PG = particles in the granulocyte region(
L/10
9
).
Having achieved the three parameters above, this analyzer proceeds to calculate the
Lymph# , Mid# and Gran# per the following equations and express them in
L/10
9
.
100
PGPMPL
PL
Lymph% ×
++
=
100
PGPMPL
PM
Mid% ×
++
=
100
PGPMPL
PG
Gran% ×
++
=
NRBC100
100
WBC'WBC
＋
＝ ×
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