Nikon A1 User Manual

Chapter 24. Capturing Large Images A1
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24.5 Specify the way of focusing
(1) Specify the way of focusing in the Focus area.
- Focus manually at start: Adjusts the focus manually at the time of start.
- Use Focus Surface: Performs automatic focusing using the Focus Surface function during
scanning of a large image.
This option is enabled when three or more points are registered in the
Focus Surface tab of the XYZ Overview window.
- Use step-by-step focus: Set the number of images after which the focus is readjusted.
- Focus manually: Adjusts display magnification so that the display area can fit the macro
screen.
Note: To use [Use Focus Surface] for focusing, set the Focus Surface in accordance with the
following procedure.
1) Select [Devices] - [Focus Surface Setup] from the menu bar to open the Focus Surface tab
of the XYZ Overview window. (Display the XYZ Overview window before opening the Scan
Large Image window.)
2) Move the XY stage and vertical Z to display a focus plane to be registered.
3) Click the [Add Point] button.
4) Repeat Steps (1) to (3) to register three or more focus planes.
(1)
A
dd Point button

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available