3-9 Adjusting the observation tube ...........................................................................................................35
1
Adjusting the interpupillary distance ........................................................................................................................35
2
Adjusting the diopter ...............................................................................................................................................................35
3
Using the eye shades ............................................................................................................................................................37
4
Adjusting the tilt ...........................................................................................................................................................................37
3-10 Adjusting the aperture diaphragm .................................................................................................38
3-11 Using the filter for reflected light illumination .......................................................................39
3-12 Setting the glare prevention ................................................................................................................ 40
3-13 Observation using the mercury burner ......................................................................................41
3-14 Using the oil immersion objective .................................................................................................. 42
4 Image acquisition ................................................................................................................................43
4-1 Image acquisition diagram ......................................................................................................................43
5 Observation methods ......................................................................................................................44
5-1 Transmitted light brightfield observation procedures ..................................................... 44
1
Notes for observation .............................................................................................................................................................46
2
Adjusting the transmitted light illumination ........................................................................................................46
3
Using the field diaphragm for transmitted light illumination ...............................................................47
4
Using the aperture diaphragm for transmitted light illumination.....................................................48
5
Using the filters for transmitted light illumination ..........................................................................................49
5-2
Reflected light differential interference contrast (DIC) observation procedures .......... 50
1
Setting the analyzer (when using U-MDICAF3) ...............................................................................................51
2
Setting the DIC slider ..............................................................................................................................................................52
3
Adjusting the prism of the DIC slider ........................................................................................................................53
5-3 Reflected light simple polarization observation procedures ...................................54
5-4 Reflected light fluorescence observation procedures ...................................................55
5-5 Reflected light IR (infrared) observation procedures .......................................................56
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