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Olympus MX63 - Reflected Light DIC Observation

Olympus MX63
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5050
5-2
Reflected light differential interference contrast (DIC) observation procedures
For this observation, the DIC mirror unit (U-MDIC3 or U-MDICAF3) must be attached to the OP position of the observation
light path by Olympus.
U-MDIC3: The analyzer and the polarizer are built-in.
U-MDICAF3: As only the polarizer is built-in, the analyzer (U-AN360-3) must be inserted to adjust the crossed nicols.
When using the mercury lamp housing, be sure to use the U-25L42 filter to prevent the polarizer from
tarnish.
NOTE
Set the main switch to (ON).
Set the DIC slider.
Set the analyzer.
Place a sample for observation on the stage.
Engage the 10X or 20X objective in the light path
using the objective selection button and
bring the sample into focus.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the brightness
(illumination voltage).
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
Observation
Adjust the brightness.
Push in the light path selection knob to the OP position
and select the DIC observation light path.
Adjust the aperture diaphragm.
[Trinocular tube only]
Push in the light path selection knob.
Main switch P.24
Observation light path selection knob P.25
Light path selection knob P.26
Light intensity knob P.32
Analyzer insertion slot P.51
DIC slider insertion slot P.52
DIC slider P.53
X-axis/Y-axis knobs P.27
Light intensity knob P.32
Adjust the prism of the DIC slider.
Operation part Page
Objective selection button P.28
Coarse focusing knob / Fine focusing
knob
P.29
Objective selection button P.28
Coarse focusing knob / Fine focusing
knob
P.29
Aperture diaphragm open/close
button
P.38
Interpupillary distance adjustment
scale
P.35
Diopter adjustment ring P.35

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