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Introduction
Scan Types
12 TSQ Quantum XLS Series User Guide Thermo Scientific
Scan Types
You can operate the TSQ Quantum XLS Series systems with a variety of scan types. The most
common scan types are as follows:
• Full Scan
• Selected Ion Monitoring (SIM)
• Selected Reaction Monitoring (SRM)
• Intelligent Selected Reaction Monitoring (iSRM)
• Quantitation-Enhanced, Data-Dependent MS (QED MS)
• AutoSIM
Full Scan
The full-scan scan type provides a full mass spectrum of each analyte. With full scan, in a
given scan time the scanning mass analyzer is scanned from the first mass to the last mass
without interruption.
You use full-scan experiments to determine or confirm the identity of unknown compounds
or the identity of each component in a mixture of unknown compounds. (Generally, you need
a full mass spectrum to determine the identity of an unknown compound.)
The full-scan scan type gives you more information about an analyte than does SIM, but a full
scan does not yield the sensitivity that SIM or SRM can achieve. With full scan, you spend
less time monitoring the signal for each ion than you do in SIM or SRM. Full scan provides
greater information but lower sensitivity than SIM or SRM scan types.
To use SIM or SRM, you must know what ions or reactions you are looking for before you
can perform an experiment with these scan types. You might use a full scan for SIM to
determine the identity of an analyte and to obtain its mass spectrum, and a full scan for SRM
to determine the mass spectrum and product mass spectra for parent ions of interest. Then,
you might use SIM or SRM to do routine quantitative analysis of the compound.
Selected Ion Monitoring (SIM)
Selected ion monitoring (SIM) monitors a particular ion or set of ions. You can perform SIM
experiments to detect small quantities of a target compound in a complex mixture when you
know the mass spectrum of the target compound. SIM is useful in trace analysis and in the
rapid screening of a large number of samples for a target compound.
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