Thermo Scientific TSQ Quantis User Manual

TSQ Series II
TSQ Altis, TSQ Quantis,
and TSQ Fortis
Hardware Manual
80111-97047 Revision A June 2018

Thermo Scientific TSQ Quantis Specifications

General

Brand	Thermo Scientific
Model	TSQ Quantis
Category	Laboratory Equipment
Language	English

Summary

Introduction

Mass Spectrometry Models

Describes the TSQ Altis, TSQ Quantis, and TSQ Fortis MS models.

MS Mass-To-Charge Ratio Ranges

Details the mass-to-charge ratio detection ranges for TSQ Altis, Quantis, and Fortis MS.

Principles of Operation

Overview of the MS System

Provides a block diagram of the MS hardware components and their functions.

Overview of Ion Optics and Mass Analyzer

Explains how ion optic assemblies and mass analyzer assemblies control ion path.

Vacuum System

Vacuum Pumps

Details the function of forepumps and turbomolecular pumps in the vacuum system.

Vacuum Manifold

Describes the vacuum manifold as a chamber enclosing MS components.

Vacuum System Block Diagram

Presents a functional block diagram of the Altis vacuum system.

Vacuum Gauges

Explains the three types of vacuum gauges used in the MS.

Atmospheric Source Interface

API Source Interface Overview

Details components of the API source interface, including sweep cone and transfer tube.

Ion Transfer Tube

Describes the ion transfer tube's function and different designs for MS systems.

RF and Exit Lens

Explains the function of RF and exit lenses in ion transmission.

Ion Optics and Detector Components

MP00 Ion Optics

Details the MP00 RF lens and L0 lens for guiding ions.

MP0 Ion Optics and Beam Blocker

Explains MP0 ion optics, beam blocker for neutral species removal.

Dual-Mode, Discrete-Dynode Ion Detection System

Describes the ion detection system including conversion dynode and electron multiplier.

Controls and Indicators

Using the MS Power Options

Turning the MS On

Procedure for turning the MS system on via the Tune window.

Placing the MS in Standby Mode

Steps to put the MS into standby mode for short-term inactivity.

Shutting Down the MS System in an Emergency

Procedure for emergency shutdown using the main power switch.

Shutting the MS System Down Completely

Shutting the MS Down Completely

Procedure for completely shutting down the MS system for extended periods.

Turning off the LC, Gases, Data System, and Autosampler

Instructions for powering down associated system components.

Restarting the MS System After a Shutdown

Restarting the Data System

Procedures for restarting the data system computer.

Resetting the Mass Spectrometer

How to reset the MS using the reset button on the communications panel.

Pumping the MS Down

Procedure for achieving sufficient vacuum after a shutdown.

Resetting Calibration Parameters

Guidance on resetting calibration parameters to factory defaults.

Starting the LC, Gases, and Autosampler

Maintaining the MS on a Daily Basis

Before Operating the Mass Spectrometer

Tasks to ensure the MS is ready for operation, including leak checks.

After Operating the Mass Spectrometer

Procedures for after-operation tasks like flushing and purging.

Recommended Inlet and Flow Rates

Guidelines for LC/H-ESI/MS Parameters

Guidelines for LC/APCI/MS Parameters

Connecting the Sample Flow to the Source

Connecting the Sample Flow to the H-ESI Source

Connecting the Sample Flow to the APCI Source

Calibrating the MS

Preparing the Syringe Pump for Calibration

Steps to prepare the syringe pump for calibration with EMRS solution.

Preparing the MS for Calibration

Procedure for setting up the MS for calibration in the Tune window.

Determining the Initial API Source Settings

Evaluating the Spray Stability

Using the Plot Chromatogram tool to assess spray stability.

Calibration Parameters

Calibrating and Tuning the MS

Procedure for successful MS calibration and tuning.

Optimizing the Compound Signal

Setting Up for Compound Optimization

Optimizing Parameters in the Optimization Pane

Steps for optimizing MS parameters in the Tune application's Optimization pane.

Scan Types

Quadrupole Capacities

Summary of Scan Types

Full Scan Q1 and Q3 Scan Types

Selected Ion Monitoring Scan Type

Product Scan Type

Precursor Scan Type

Neutral Loss Scan Type

Selected Reaction Monitoring Scan Type

Data Types

Profile and Centroid Data

Ion Polarity Modes

Acquiring Sample Data

Using Tune to Acquire Sample Data

Using the Xcalibur Data System to Acquire Sample Data

Using Templates in Thermo Xcalibur Instrument Setup

Maintenance Schedule and Supplies

MS Parts

Maintenance Schedule

Details maintenance procedures and their recommended frequency.

Tools and Supplies

Maintaining the API Source and Replacing the H-ESI Needle

Cleaning the API Source Housing

Replacing the H-ESI Needle

Maintaining the RF Lens, Exit Lens, MP00 RF Lens, and Lens L0

Removing the API Source Interface

Removing the RF Lens, Exit Lens, MP00 RF Lens, and Lens L0 (Altis/Quantis)

Removing the Exit Lens, MP00, and Lens L0 (Fortis)

Cleaning the RF Lens, Exit Lens, MP00, and Lens L0

Reinstalling the API Source Interface

Maintaining the API Source Interface

Removing the Ion Sweep Cone and the Ion Transfer Tube

Cleaning the Spray Cone and O-ring

Cleaning the Ion Transfer Tube

Cleaning the Ion Sweep Cone

Maintaining the Forepumps and the Air Filter

Maintaining the Forepumps

Maintaining the Air Filter

Appendix A: Using Basic Tune Functions

Setting the Instrument System Controls

Setting the Instrument Power Mode

Checking the Instrument Readback Status

Setting the Tune Preferences

Using the Mass List Table in the Define Scan Pane

Using the Favorites Pane to Save System Settings

Appendix B: Setting Up the Syringe with the Syringe Pump

Connecting the Syringe Union to the Syringe

Setting Up the Syringe Pump

How to use the syringe pump for sample infusion or loading.

Appendix C: Divert/Inject Valve

About the Divert/Inject Valve

Divert/Inject Valve Configurations

Loop Injection

Divert Valve

Divert/Inject Valve Controls

Appendix D: Setting Up Sample Introduction Techniques

Setting Up H-ESI or Low Flow H-ESI Direct Infusion

Technique for calibrating and infusing samples using syringe pump.

Setting Up H-ESI Direct Infusion

Setting Up Low Flow H-ESI Direct Infusion

Setting Up High-Flow Infusion Without an Autosampler

Setting Up Loop Injections for Flow-Injection Analysis

Setting Up Injections Using the Autosampler