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Calibrating the MS
Determining the Initial API Source Settings
Thermo Scientific TSQ Altis, Quantis, and Fortis Hardware Manual 57
Figure 25. Syringe pump settings box
c. Click Apply.
5. Verify that the inlet plumbing connections do not leak.
6. Open the syringe pump settings box again. Press and hold Prime to prime the syringe at
100 μL/min.
7. Verify that the system readback is normal, .
Determining the Initial API Source Settings
These initial setting are a starting point for calibrating and optimizing system performance.
1. In the Tune window, click the Ion Source tab.
2. In the Current LC Flow (μL/min) box, type the flow rate from Table 11, and then click
Get Defaults.
3. Click Apply.
The Tune application makes a change in the History pane.
Table 11. Recommended LC flow rates
Solution Type Injection Type LC flow rate (μL/min)
Calibration Syringe 5
Sample Syringe 3-10
Autosampler 200