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Calibrating the MS
Calibrating and Tuning the MS
Thermo Scientific TSQ Altis, Quantis, and Fortis Hardware Manual 61
5. Click Calibration to display the Calibration Status page, and then click Calibrate to
display the options in the Calibration pane (Figure 28).
Ensure that the calibration ions are present in the real-time spectrum. If the syringe was
not primed, it may take a few minutes. For a list of the calibration ions, see “Calibration
Solution Peak Values.”
Figure 28. Calibration pane options
6. Select one of the Calibration options. Before you run each of these options the system
evaluates the spray stability.
• Check Mass Calibration
• Mass Calibration
• EM Gain Calibration
• Tune and Mass Calibration
7. Click Start.
When the calibration is complete, the Tune application prompts you to generate a report
of the calibration results.
Figure 29. Report Generation Options
8. Select an option and click OK.
9. After the MS completes the calibration procedure in positive mode, run the calibration
procedures in negative ion mode.
IMPORTANT Check the spray and reevaluate it when it becomes unstable. If the spray
is not stable, then data quality might be compromised, which can result in a poor
calibration or diagnostic result.
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