15
Calibrating the MS
Preparing the MS for Calibration
56 TSQ Altis, Quantis, and Fortis Hardware Manual Thermo Scientific
1. Load a clean, 500 μL syringe with EMRS.
Use the following information to determine how much EMRS to use.
• Tuning and calibrating take 30–50 minutes.
• A full 500 μL syringe delivers sample for 100 minutes at a flow rate of 5 μL/min.
Use a full syringe the first time you tune and calibrate.
2. Turn on the syringe pump’s power switch (on the back of the device).
3. In the Tune window, place the MS in Standby mode.
Preparing the MS for Calibration
1. In the Tune window, place the MS in On mode, .
2. Click Profile (Centroid) and select the profile data type.
3. In the Favorites pane under System Settings, right-click Default for EMRS Calibration
with Positive Polarity, and choose Apply.
The default parameter settings appear at the top of the Favorites pane.
4. Set the syringe pump parameters as follows:
a. Click Syringe Off to turn on the syringe pump.
The button name changes to Syringe On.
b. Click the arrow next to the Syringe On/Off button to open the syringe pump settings
box (Figure 25), and then enter the following:
Flow Rate (μL/min): 5
Volume (μL): 500
IMPORTANT Observe these storage precautions for EMRS and reserpine solutions:
• Refrigerate the containers after opening.
• For long-term storage, keep the containers refrigerated at 2-8 °C (36-46 °F).
CAUTION To prevent electric shock, verify that the grounding union is made of
stainless steel. A grounding union made of a non-conductive material, such as PEEK,
creates an electric shock hazard.
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