• Before the CO
2
concentration is validated, the EmbryoScope time-lapse incubator must
have been turned on for at least two hours with the appropriate gasses connected to
ensure that full equilibrium is attained. The room temperature must be equivalent to
normal laboratory temperature.
• Before you start, make sure that the gas analyser is calibrated according to the
manufacturer’s specifications.
1. Switch on the gas analyser used for measuring the CO
2
concentration.
2. Remove the service lid on the EmbryoScope time-lapse incubator.
3. Attach the tube from the gas analyser to the gas sample pipe.
4. Open the valve by turning the lever towards you until the lever is in upright position.
5. Press Check on the touch screen of the EmbryoScope time-lapse incubator.
6. Measure the CO
2
concentration two or three times or until the measurement is stable.
7. If the measured CO
2
concentration deviates from the set point, you need to calibrate the CO
2
concentration as described in section 5.5.3.
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